منابع مشابه
Cloning of bovine CD34 cDNA.
CD34 is a leukocyte antigen that is expressed in various cell types including hematopoietic cells. Monoclonal antibodies against human, murine, and canine CD34 proteins have been used for the identification of lymphohematopoietic stem/progenitor cells. The cDNA encoding bovine CD34 was cloned, and its nucleotide sequence was determined. The identity of the deduced amino acid sequence of the enc...
متن کاملMolecular cloning of a bovine immunoglobulin lambda chain cDNA.
A cDNA library of the bovine mammary gland constructed in pBR322 was screened by mRNA hybrid-selected translation and by differential hybridization. Several immunoglobulin (Ig) lambda light-chain clones were identified and sequenced. Nucleotide sequence comparison of bovine and human Ig lambda chains showed a high degree of homology for constant regions and for J regions. The amino acid (aa) se...
متن کاملRapid communication: cloning of bovine serum amyloid A3 cDNA.
Name of Sequence. Bovine serum amyloid A3. Genus and Species, Breed. Bos taurus, Holstein. Origin of Clone. cDNA clones that showed differentially up-regulated message in involuted mammary tissues compared with lactating tissues have been isolated by a subtractive suppression hybridization method. These clones were sequenced by a dideoxy method using ABI PRISM Big Dye Terminators (Forster City,...
متن کاملCloning and analysis of a cDNA coding for bovine prothrombin.
Poly(A)-RNA enriched for prothrombin was isolated by specific immunoprecipitation of bovine liver polysomes. Prothrombin consisted of about 8% of the cell-free translation products of this RNA. A double-stranded cDNA was synthesized by using reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) and made blunt-ended with nuclease S1. After tailing with dCTP and terminal transferase, t...
متن کاملCanine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein.
Increasingly, enriched populations of hematopoietic progenitors are used in experimental and clinical transplantation studies. The separation of progenitors is based on the expression of CD34, a marker preferentially expressed on progenitor cells. The dog model has been important for preclinical transplant studies, because it has proven predictive for outcomes in human hematopoietic stem cell t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2001
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.63.1051